《植物生理学报》 2018, 54(9): 1441-1450

锯缘落地生根的器官特征及多梳蛋白KsRING1的组织表达分析

屈东海¹, 仇汉林², 黄勇¹, 阮颖¹, 陈东红^2,*

¹湖南农业大学生物科学技术学院, 长沙410128; ²浙江农林大学亚热带森林培育国家重点实验室, 浙江临安311300

通信作者：陈东红；E-mail: chendh212@163.com

摘　要：

在拟南芥中, 多梳蛋白抑制复合体1 (PRC1)的核心组分AtRING1作为重要的表观遗传因子, 其功能缺失导致植物在营养阶段产生胚性愈伤结构, 这与落地生根叶生无性苗的发生相类似。本研究首先系统观察了锯缘落地生根主要器官的结构特征, 探讨了锯缘落地生根整体发育模式。然后通过同源克隆技术获得2个AtRING1在锯缘落地生根的同源基因并命名为KsRNG1a和KsRING1b, GenBank登录号分别为MH144360和MH144361。实时定量PCR结果表明, KsRING1a/b基因广泛表达于锯缘落地生根的不同组织器官中, 表明其功能贯穿于植物生长发育的整个时期。特别是KsRING1a/b的表达量在叶缘无性苗中的表达最高, 这与拟南芥同源基因AtRING1a/b在种子胚中的高表达一致, 不同于AtRING1突变导致的体细胞胚性结构发生, 这表明锯缘落地生根叶缘无性苗的发生过程更接近于常规的种子胚发生方式。

关键词：锯缘落地生根; 器官发育; 无性苗; 多梳蛋白; KsRING1

收稿：2018-05-30   修定：2018-09-11

资助：浙江农林大学科研发展基金人才启动项目(102/20340-20128)和国家重点研发课题(2017YFC1702201)。

Organ characteristics and expression analysis of polycomb subunit KsRING1 in Kalanchoe serrata

QU Dong-Hai¹, QIU Han-Lin², HUANG Yong¹, RUAN Ying¹, CHEN Dong-Hong^2,*

¹ College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; ² State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, Zhejiang 311300, China

Corresponding author: CHEN Dong-Hong; E-mail: chendh212@163.com

Abstract:

In model plant Arabidopsis thaliana, AtRING1 is the core subunit of the Polycomb repressive complex 1 (PRC1) and acts as an important epigenetic factor; its knockout results in the production of embryogenic callus during the vegetative stage, somewhat similar to the emergence of asexual seedlings in K. serrata. In this study, we first systematically analyzed the structural characteristics of the main organs and explored the overall development pattern in K. serrata. Two AtRING1 homologous genes in K. serrata were then obtained by homologous cloning method and named as KsRNG1a and KsRING1b with GenBank accession numbers MH144360 and MH144361, respectively. Quantitative PCR (qPCR) showed that the KsRING1a/b genes were widely expressed in different tissues and organs in K. serrata, indicating that the function of KsRING1a/b was throughout the entire period of plant growth and development. In particular, the expression level of KsRING1a/b was highest in asexual seedlings of leaf margin, which was consistent with the high expression of Arabidopsis AtRING1 homologues in seed embryos but was different from AtRING1 depletion resulting in the somatic embryogenic structure. This suggests that the occurrence of asexual seedlings in the leaf margin of K. serrata is more similar to the seed embryogenesis pattern in normal plants.

Key words: Kalanchoe serrata; organogenesis; asexual seedlings; polycomb; KsRING1